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mouse embryonic fibroblast  (ATCC)


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    ATCC mouse embryonic fibroblast
    ( A ) Mouse embryonic <t>fibroblasts</t> (MEFs) were co-transfected with plasmids for expression of FAK 379-408 -2A-DsRed and FAK-GFP. After 24 hours the cells were seeded onto poly-D-Lysine plates and imaged for DNA (Hoechst), DsRed, and GFP fluorescence. ( B ) DNA, FAK 379-408 , and FAK imaging. Cells expressing significant FAK-GFP but not FAK 379-408 form condensates; cells expressing both FAK-GFP and FAK 379-408 exhibit drastic reduction of FAK condensates. ( C ) Representative cell expressing FAK-GFP only vs. cell expressing FAK-GFP + FAK 379-408 -2A-DsRed, at the same FAK transfection level (GFP intensity of ∼250 a.u.; Methods ). ( D ) Quantification of microscopy results. Number of FAK condensate puncta ( N condensates ) per cell as a function of the fragment:FAK (DsRed:GFP) intensity ratio in co-transfected cells. The number of puncta is negatively correlated with the intensity ratio (Spearman ρ = −0.54, P < 10 −10 ). ( E ) Bar plot from data in (D), comparing number of condensates per cell for fragment:FAK intensity ratios of ≤ 0.5 and ≥ 1. Bar height, mean value; error bars, standard deviation. *, difference is significant at p < 0.05, Student’s t-test.
    Mouse Embryonic Fibroblast, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+embryonic+fibroblast/bio_rxiv__64898__2026__05__08__723928-202-0-4?v=ATCC
    Average 93 stars, based on 124 article reviews
    mouse embryonic fibroblast - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "AI-discovered protein fragments as generalizable regulators of biomolecular condensates"

    Article Title: AI-discovered protein fragments as generalizable regulators of biomolecular condensates

    Journal: bioRxiv

    doi: 10.64898/2026.05.08.723928

    ( A ) Mouse embryonic fibroblasts (MEFs) were co-transfected with plasmids for expression of FAK 379-408 -2A-DsRed and FAK-GFP. After 24 hours the cells were seeded onto poly-D-Lysine plates and imaged for DNA (Hoechst), DsRed, and GFP fluorescence. ( B ) DNA, FAK 379-408 , and FAK imaging. Cells expressing significant FAK-GFP but not FAK 379-408 form condensates; cells expressing both FAK-GFP and FAK 379-408 exhibit drastic reduction of FAK condensates. ( C ) Representative cell expressing FAK-GFP only vs. cell expressing FAK-GFP + FAK 379-408 -2A-DsRed, at the same FAK transfection level (GFP intensity of ∼250 a.u.; Methods ). ( D ) Quantification of microscopy results. Number of FAK condensate puncta ( N condensates ) per cell as a function of the fragment:FAK (DsRed:GFP) intensity ratio in co-transfected cells. The number of puncta is negatively correlated with the intensity ratio (Spearman ρ = −0.54, P < 10 −10 ). ( E ) Bar plot from data in (D), comparing number of condensates per cell for fragment:FAK intensity ratios of ≤ 0.5 and ≥ 1. Bar height, mean value; error bars, standard deviation. *, difference is significant at p < 0.05, Student’s t-test.
    Figure Legend Snippet: ( A ) Mouse embryonic fibroblasts (MEFs) were co-transfected with plasmids for expression of FAK 379-408 -2A-DsRed and FAK-GFP. After 24 hours the cells were seeded onto poly-D-Lysine plates and imaged for DNA (Hoechst), DsRed, and GFP fluorescence. ( B ) DNA, FAK 379-408 , and FAK imaging. Cells expressing significant FAK-GFP but not FAK 379-408 form condensates; cells expressing both FAK-GFP and FAK 379-408 exhibit drastic reduction of FAK condensates. ( C ) Representative cell expressing FAK-GFP only vs. cell expressing FAK-GFP + FAK 379-408 -2A-DsRed, at the same FAK transfection level (GFP intensity of ∼250 a.u.; Methods ). ( D ) Quantification of microscopy results. Number of FAK condensate puncta ( N condensates ) per cell as a function of the fragment:FAK (DsRed:GFP) intensity ratio in co-transfected cells. The number of puncta is negatively correlated with the intensity ratio (Spearman ρ = −0.54, P < 10 −10 ). ( E ) Bar plot from data in (D), comparing number of condensates per cell for fragment:FAK intensity ratios of ≤ 0.5 and ≥ 1. Bar height, mean value; error bars, standard deviation. *, difference is significant at p < 0.05, Student’s t-test.

    Techniques Used: Transfection, Expressing, Fluorescence, Imaging, Microscopy, Standard Deviation



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    Image Search Results


    ( A ) Mouse embryonic fibroblasts (MEFs) were co-transfected with plasmids for expression of FAK 379-408 -2A-DsRed and FAK-GFP. After 24 hours the cells were seeded onto poly-D-Lysine plates and imaged for DNA (Hoechst), DsRed, and GFP fluorescence. ( B ) DNA, FAK 379-408 , and FAK imaging. Cells expressing significant FAK-GFP but not FAK 379-408 form condensates; cells expressing both FAK-GFP and FAK 379-408 exhibit drastic reduction of FAK condensates. ( C ) Representative cell expressing FAK-GFP only vs. cell expressing FAK-GFP + FAK 379-408 -2A-DsRed, at the same FAK transfection level (GFP intensity of ∼250 a.u.; Methods ). ( D ) Quantification of microscopy results. Number of FAK condensate puncta ( N condensates ) per cell as a function of the fragment:FAK (DsRed:GFP) intensity ratio in co-transfected cells. The number of puncta is negatively correlated with the intensity ratio (Spearman ρ = −0.54, P < 10 −10 ). ( E ) Bar plot from data in (D), comparing number of condensates per cell for fragment:FAK intensity ratios of ≤ 0.5 and ≥ 1. Bar height, mean value; error bars, standard deviation. *, difference is significant at p < 0.05, Student’s t-test.

    Journal: bioRxiv

    Article Title: AI-discovered protein fragments as generalizable regulators of biomolecular condensates

    doi: 10.64898/2026.05.08.723928

    Figure Lengend Snippet: ( A ) Mouse embryonic fibroblasts (MEFs) were co-transfected with plasmids for expression of FAK 379-408 -2A-DsRed and FAK-GFP. After 24 hours the cells were seeded onto poly-D-Lysine plates and imaged for DNA (Hoechst), DsRed, and GFP fluorescence. ( B ) DNA, FAK 379-408 , and FAK imaging. Cells expressing significant FAK-GFP but not FAK 379-408 form condensates; cells expressing both FAK-GFP and FAK 379-408 exhibit drastic reduction of FAK condensates. ( C ) Representative cell expressing FAK-GFP only vs. cell expressing FAK-GFP + FAK 379-408 -2A-DsRed, at the same FAK transfection level (GFP intensity of ∼250 a.u.; Methods ). ( D ) Quantification of microscopy results. Number of FAK condensate puncta ( N condensates ) per cell as a function of the fragment:FAK (DsRed:GFP) intensity ratio in co-transfected cells. The number of puncta is negatively correlated with the intensity ratio (Spearman ρ = −0.54, P < 10 −10 ). ( E ) Bar plot from data in (D), comparing number of condensates per cell for fragment:FAK intensity ratios of ≤ 0.5 and ≥ 1. Bar height, mean value; error bars, standard deviation. *, difference is significant at p < 0.05, Student’s t-test.

    Article Snippet: Mouse embryonic fibroblast (MEF ATCC CRL-2645) cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM; high glucose, Invitrogen) supplemented with 10% fetal bovine serum (FBS, Thermo Scientific), 1% penicillin–streptomycin, and 2 mM Glutamax at 37°C in a humidified incubator with 5% CO2.

    Techniques: Transfection, Expressing, Fluorescence, Imaging, Microscopy, Standard Deviation